Figure 2.

Confirmation of hd2 mutants and drought stress-induced changes in the hd2 mutant lines. (A) Genotyping of double mutant line Mac16 for HD2A and HD2C genes. Gene-specific left (LP) and right (RP) primers along with TDNA primers GK8474 and LBa1 were used in genotyping. (B) Relative expression of HD2A and HD2C in WT, the single mutants hd2a and hd2c, and the double mutant Mac16. Data shown are means ± standard errors (n = 3). (**p < 0.01). (C) Images of WT, hd2a, hd2c, and Mac16 plants under control and two days after rewatering, following 11 days of drought stress. (D) Survival rates of hd2a, hd2c, and Mac16 relative to WT plants after drought stress. Data shown are means ± standard errors (n = 3) from three independent experiments. In each experiment, at least 30 plants of each genotype were used to calculate the survival. The significance of the differences between different genotypes was determined by one-way ANOVA followed by post-hoc Tukey’s HSD tests. Lowercase letters indicate significant differences (p < 0.01). (E) Fresh leaf water loss measured in WT, hd2a, hd2c, and Mac16 at every 30-minute interval for the period of 180 minutes. Data shown are means ± standard errors (n = 3) from three independent experiments. In each experiment, measurements were taken from three plants (three leaves per plant).