Figure 4.

Stomatal closure under stress in HD2A and HD2C overexpression lines. Images of stomatal pore opening and closure observed in WT, HD2A-OE line (A) and HD2C-OE line (C) under control and ABA-induced stress (scale bar: 50 µm). (B) Stomatal aperture measured as width/length of stomatal pores in WT, HD2A-OE lines, OEA1 and OEA2 (B) and HD2C-OE lines, OEC1 and OEC2 (D) under control and ABA-induced stress. Data shown are means ± standard errors from at least 150 stomata. Three independent experiments were performed in this test. In each experiment, stomatal measurements were taken from three plants of each genotype (two leaves per plant). The significance of the differences between different genotypes was determined by Student’s t test (**p < 0.01).