Figure 5.

Stomatal closure and gene expression analysis under stress in hd2 mutant lines. (A) Images of stomatal pore opening and closure observed in WT, hd2a, hd2c, and Mac16 under control and ABA-induced stress (scale bar: 50 µm). (B) Stomatal aperture measured as width/length of stomatal pores in WT, hd2a, hd2c, and Mac16 under control and ABA-induced stress. Data shown are means ± standard errors from at least 150 stomata. Three independent experiments were performed in this test. In each experiment, stomatal measurements were taken from three plants of each genotype (two leaves per plant). (C, D) Relative expression of ABI1 and ABI2 (C) and SLAC1 (D) in WT, hd2a, hd2c, and Mac16 under drought conditions. Data shown are means ± standard errors (n = 3). (B-D) The significance of the differences between different genotypes was determined by one-way ANOVA followed by post-hoc Tukey’s HSD tests. Lowercase letters indicate significant differences (p < 0.01).