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. 2022 Nov 23;13:993498. doi: 10.3389/fphar.2022.993498

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Copyright © 2022 Qiu, Zhang, Li, Hou, Liu, Lin, Yao, Duan, Zhang, Li, Li, Wang, Li, Jin and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Culture and identification of rat chondrocytes, and the effects of seven potential active constituents (Isoliquiritigenin, Emodin, Taxifolin, Catalpol, Alisol A, Zingerone, and Schisandrin C) on rat chondrocytes viability. (A1) Primary rat chondrocytes cultured for 24 h; (A2) Primary rat chondrocytes cultured for 7 days; (A3) Toluidine blue staining of primary rat chondrocytes; (A4) Immunofluorescence staining of collagen II of primary rat chondrocytes; (B) Effect of seven potential active constituents (Isoliquiritigenin, Emodin, Taxifolin, Catalpol, Alisol A, Zingerone, and Schisandrin C) on chondrocytes viability at different concentrations (0, 5, 10, 20, 40, and 80 μM) for 24 h. *p < 0.05, **p < 0.01 compared with the control group.