Table 1.
Description of a select subset of transcription factors that are either regulated by EWS::FLI1 or play an important role in altering expression of EWS::FLI1 targets.
| TF | Regulated by EWS::FLI1? | Direct EWS::FLI1 target? | Effect on EWS::FLI1 | Effect on EWS::FLI1 targets |
|---|---|---|---|---|
| MEIS1 (79) | Upregulated by EWS::FLI1 | NR | NR | Augments EWS::FLI1 activated signature |
| RUNX3 (80) | EWS::FLI1 inhibits RUNX3 activity | NR | Binds EWS::FLI1 | De-represses genes downregulated by EWS::FLI1 |
| FEZF1 (81) | Upregulated by EWS::FLI1 | Yes | No effect on EWS::FLI1 expression | Augments EWS::FLI1 signature, particularly neural genes |
| E2F3 (82, 83) | Upregulated by EWS::FLI1 | NR | NR | Augments EWS::FLI1 signature |
| NKX2-2 (30, 47, 79) | Upregulated by EWS::FLI1 | Yes | No effect on EWS::FLI1 expression | Augments EWS::FLI1 repressed signature |
| HOXD13 (22) | Upregulated by EWS::FLI1 | Yes | No effect on EWS::FLI1 expression | De-represses genes downregulated by EWS::FLI1 |
| ZEB2 (30, 90) | Expression not regulated by EWS::FLI1 | No | No effect on EWS::FLI1 expression | De-represses genes downregulated by EWS::FLI1 |
| NR0B1 (25, 91, 172) | Upregulated by EWS::FLI1 | Yes | Binds EWS::FLI1 | Augments EWS::FLI1 signature |
| BCL11B (92, 173) | Upregulated by EWS::FLI1 | Yes | NR | Augments EWS::FLI1 repressed signature |
| GLI1 (83, 93, 174) | Upregulated by EWS::FLI1 | Yes | NR | Augments EWS::FLI1 signature |
| SOX2 (36, 38, 51) | Upregulated by EWS::FLI1 | Yes, and regulated by miRNA-145 | No effect on EWS::FLI1 expression | Augments EWS::FLI1 signature |
| FOXO1 (98, 175) | Downregulated by EWS::FLI1 | Yes | No effect on EWS::FLI1 expression | De-represses genes downregulated by EWS::FLI1 |
NR, not reported.