Figure 1.

Cell Viability and Cytotoxic Effect of Asp and NNDAsp Treatment. (A) Cell viability rate of Caco-2 cells that were pretreated with indicated concentrations of either Asp or NNDAsp for 24 hrs compared with DMSO (0 concentration). (B) Cell viability rate of NCM-460 cells that were pretreated with the same concentration of Asp or NNDAsp for 24 hrs compared with DMSO (0 concentration) using MTT assay. (C) Relative LDH production from treated cells compared to Triton 100-X treated cells and untreated cells (N.T.). Error bars indicate the S.D. of four different replicates. The student's two tails t-test was used to determine the significance of differentiated values. (*) indicates P-values ≤0.05, and (**) indicates the P ≤0.01