Figure 4.

Expression Profile ASNS Protein in Amino Acid-Treated Cells. The concentration of ASNS (pm/ml) in Caco-2 cells treated with different concentrations of each amino acid for 24 hrs compared with DMSO-treated cells. (B) The concentration of ASNS (pm/ml) in Caco-2 cells treated with 4 mg/ml of each amino acid and incubated for the indicated time point, DMSO-treated cells severed as control treatment. Error bars reveal the S.D. of three replicates. (C) Flow cytometry fractionation quantifies the kinetic profile of ASNS protein expression in Caco-2 cells treated with 4 mg/ml of each amino acid compared to DMSO treated cells