Figure 2.

Proliferation of CD8+ T-cells Isolated from CLL Patients after PD-1 and TIGIT Blockade. CD8+ T-cells separated from CLL patients were treated with anti-PD-1 and anti-TIGIT blocking antibodies and then co-cultured with malignant B-cells as target cells. Cells were then stimulated with anti-CD3/CD28 antibodies and cell proliferation was evaluated using the MTT assay. Stimulation index (SI) was calculated by dividing the OD values of cells treated with blocking antibodies or their isotype controls into the OD value of blank