Figure 3.

Degranulation of T-CD8 + T-cells Isolated from CLL Patients after PD-1 and TIGIT Blockade. CD8+ T-cells separated from CLL patients were treated with anti-PD-1 and anti-TIGIT blocking antibodies and then co-cultured with malignant B-cells as target cells. Cells were then stimulated with PMA/ ionomycin and evaluated using a two-color flow cytometric analysis with anti-CD107a-PE and anti-CD8-FITC antibodies. A) Representative graphs showing the percentage of CD8+CD107a+ cells obtained from a CLL patient. B) CD107a expression using a combined blockade of PD-1/TIGIT, single blockade of PD-1, single blockade of TIGIT, and the isotype control antibody