Figure 2.

Iranian Mesobuthus Eupeus Crude Venom Induced the Formation of ROS in CLL B-Lymphocytes. (A), The fluorescent intensity of DCF was detected by flow cytometry in the isolated CLL B-lymphocytes treated with different concentrations of IMe crude venom for 12 hours. Histograms showed a considerable shift to the right in the isolated CLL B-lymphocytes; (B), Quantification of ROS formation in the isolated CLL B-lymphocytes was presented in the graph. The values are the mean ± SD of three independent experiments (n=3). Significance was determined using one-way ANOVA. ***p < 0.001. IMe, Iranian Mesobuthus eupeus; ROS, Reactive Oxygen Species; DCF-DA, Dichlorofluorescin Diacetate; FCS, Flow Cytometry Standard