Figure 3.

Iranian Mesobuthus Eupeus Crude Venom Induced Mitochondrial Membrane Potential Depolarization in CLL B-lymphocytes. (A), The isolated CLL B-lymphocytes treated with 40, 60 and 80 μg/ml IMe crude venom for 12 hours. Mitochondrial membrane potential depolarization effects were detected using cell-permeable cationic rhodamine 123 and flow cytometry. Intensity of mitochondrial membrane potential depolarization-disrupted in the isolated CLL B-lymphocytes significantly increased with increasing concentrations of IMe crude venom (40, 60 and 80 μg/ml); (B), Quantification of mitochondrial membrane potential depolarization in the isolated CLL B-lymphocytes was presented in the graph. The values are the mean ± SD of three independent experiments (n=3). Significance was determined using one-way ANOVA. ***p < 0.001. IMe, Iranian Mesobuthus eupeus; FCS, Flow Cytometry Standard