Figure 4.

Iranian Mesobuthus Eupeus Crude Venom Induced the Lysosomal Membrane Destabilization in CLL B-lymphocytes. (A), The fluorescent intensity of acridine orange was detected by flow cytometry in the isolated CLL B-lymphocytes treated with different concentrations of IMe crude venom for 12 hours; (B), Quantification of lysosomal membrane destabilization in the isolated CLL B-lymphocytes was presented in the graph. The values are the mean ± SD of three independent experiments (n=3). Significance was determined using one-way ANOVA. ***p < 0.001. IMe, Iranian Mesobuthus eupeus; FCS, Flow Cytometry Standard; AO, Acridine Orange