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. 2022 Aug 2;18(3):634–642. doi: 10.4103/1673-5374.350210

Figure 6.

Figure 6

Neuroinflammation at the L2/3 level caused by the absence of SPARC.

(A–C) Immunofluorescence staining for Iba1 (green) with IL-1β, IL-6, and CD206 (red). The levels of Iba1, IL-1β, and IL-6 with Iba1 (markers of M1 phenotype microglia/macrophages) were significantly increased in SPARC-null mice compared with WT control. (D) Immunofluorescence staining for CD3 and CD11b (red). Scale bar: 20 µm. (E) Western blot and quantitative analysis of iNOS, CD206, IL-1β, IL-6, and TNF-α (normalized to β-actin). Data are shown as mean ± SD (n = 3 mice in each group). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). Iba1: Ionized calcium binding adapter molecule 1; IL: interleukin; iNOS: inducible nitric oxide synthase; SPARC: secreted protein, acidic and rich in cysteine; TNF-α: tumor necrosis factor-α; WT: wild-type.