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. 2022 Aug 2;18(3):634–642. doi: 10.4103/1673-5374.350210

Figure 8.

Figure 8

The absence of SPARC activates NLRP3 inflammasomes at the L2/3 level in spinal cord.

(A) Immunofluorescence staining of Iba1 (green) with NLRP3 (red). NLRP3 expression was low in WT mice, while it was enhanced in neurons of SPARC-null mice, especially in the compression part. (B) Immunofluorescence staining of βIII-tubulin (green) with NLRP3 (red). NLRP3 expression was low in WT mice, while it was enhanced in microglia of SPARC-null mice, especially in the compression part. Scale bars: 20 µm. (C) Western blot and quantitative analysis of NLRP3, ASC, pro caspase-1, and cleavage caspase-1 (normalized to β-actin). Data are shown as mean ± SD (n = 3 mice in each group). **P < 0.01, ****P < 0.0001 (Student’s t-test). ASC: Apoptosis-associated speck-like protein containing a CARD; Iba1: ionized calcium binding adapter molecule 1; NLRP3: NOD-like receptor protein 3; SPARC: Secreted protein, acidic and rich in cysteine; WT: wild-type.