. 2022 Aug 2;18(3):671–682. doi: 10.4103/1673-5374.350214

Table 3.

Sequences of rat miR-221 and mcherry-miR-338 gene fragments

Name	Sequence
Pre-miR-221*	5’-ACC GGT TGG TAA GTC GCC ACA ATT GTG ACT TTT GTT TTC TTT TCT TTA TGG GAA ATA AGA CAC AAA CCA ACT TTG ACT TGT TCA TCT GTG TGG ATA GTT TGC AGG CTG AAT ATC CAG GTC TGG GGC ATG AAC CTG GCA TAC AAT GTA GAT TTC TGT GTT TGT TAG GCA ACA GCT ACA TTG TCT GCT GGG TTT CAG GCT ACC TGG AAG CAT TTC TCC ATT GGC CCT CTT ATC AAT GTT TTA ACT CTA ATA TTT GTG AGT CAG ATC TTC TGG GAG CTA TAA ATG GGT GTG ACA TAT CTT ATC GGA AAG AAC AAA AGA GCT AGC-3’
mCherry-pre-miR-338**	5’-ACC GGC GCC ACC ATG GTG AGC AAG GGC GAG GAG GAT AAC ATG GCC ATC ATC AAG GAG TTC ATG CGC TTC AAG GTG CAC ATG GAG GGC TCC GTG AAC GGC CAC GAG TTC GAG ATC GAG GGC GAG GGC GAG GGC CGC CCC TAC GAG GGC ACC CAG ACC GCC AAG CTG AAG GTG ACC AAG GGT GGC CCC CTG CCC TTC GCC TGG GAC ATC CTG TCC CCT CAG TTC ATG TAC GGC TCC AAG GCC TAC GTG AAG CAC CCC GCC GAC ATC CCC GAC TAC TTG AAG CTG TCC TTC CCC GAG GGC TTC AAG TGG GAG CGC GTG ATG AAC TTC GAG GAC GGC GGC GTG GTG ACC GTG ACC CAG GAC TCC TCC CTG CAG GAC GGC GAG TTC ATC TAC AAG GTG AAG CTG CGC GGC ACC AAC TTC CCC TCC GAC GGC CCC GTA ATG CAG AAG AAG ACC ATG GGC TGG GAG GCC TCC TCC GAG CGG ATG TAC CCC GAG GAC GGC GCC CTG AAG GGC GAG ATC AAG CAG AGG CTG AAG CTG AAG GAC GGC GGC CAC TAC GAC GCT GAG GTC AAG ACC ACC TAC AAG GCC AAG AAG CCC GTG CAG CTG CCC GGC GCC TAC AAC GTC AAC ATC AAG TTG GAC ATC ACC TCC CAC AAC GAG GAC TAC ACC ATC GTG GAA CAG TAC GAA CGC GCC GAG GGC CGC CAC TCC ACC GGC GGC ATG GAC GAG CTG TAC AAG TAA GCG GCC GCC AGG CCA GGA TGT CCA GTC GCC CGT GAG CCG GGG GGC ACG GGC TGG AAG AAG TAG CGA TGG ACG AGC GGC CCG CCA GCC CCG ACG CTG CCC AGG CCG TCC TCC CCA ACA ATA TCC TGG TGC TGA GTG GGT GCA CAG TGA CTC CAG CAT CAG TGA TTT TGT TGA AGA GGG CAG CTG CCA GCC TCC TGT CCT GCC TGA TGC CTA GCC CAG CTG CCC CAC CCA TAG GTC ACC CAA CCC CGC ATC CGT CCC CTG GTA CAG TCC TCC TGG GAC GCG T -3’

Name

Sequence

Pre-miR-221*

5’-ACC GGT TGG TAA GTC GCC ACA ATT GTG ACT TTT GTT TTC TTT TCT TTA TGG GAA ATA AGA CAC AAA CCA ACT TTG ACT TGT TCA TCT GTG TGG ATA GTT TGC AGG CTG AAT ATC CAG GTC TGG GGC ATG AAC CTG GCA TAC AAT GTA GAT TTC TGT GTT TGT TAG GCA ACA GCT ACA TTG TCT GCT GGG TTT CAG GCT ACC TGG AAG CAT TTC TCC ATT GGC CCT CTT ATC AAT GTT TTA ACT CTA ATA TTT GTG AGT CAG ATC TTC TGG GAG CTA TAA ATG GGT GTG ACA TAT CTT ATC GGA AAG AAC AAA AGA GCT AGC-3’

mCherry-pre-miR-338**

5’-ACC GGC GCC ACC ATG GTG AGC AAG GGC GAG GAG GAT AAC ATG GCC ATC ATC AAG GAG TTC ATG CGC TTC AAG GTG CAC ATG GAG GGC TCC GTG AAC GGC CAC GAG TTC GAG ATC GAG GGC GAG GGC GAG GGC CGC CCC TAC GAG GGC ACC CAG ACC GCC AAG CTG AAG GTG ACC AAG GGT GGC CCC CTG CCC TTC GCC TGG GAC ATC CTG TCC CCT CAG TTC ATG TAC GGC TCC AAG GCC TAC GTG AAG CAC CCC GCC GAC ATC CCC GAC TAC TTG AAG CTG TCC TTC CCC GAG GGC TTC AAG TGG GAG CGC GTG ATG AAC TTC GAG GAC GGC GGC GTG GTG ACC GTG ACC CAG GAC TCC TCC CTG CAG GAC GGC GAG TTC ATC TAC AAG GTG AAG CTG CGC GGC ACC AAC TTC CCC TCC GAC GGC CCC GTA ATG CAG AAG AAG ACC ATG GGC TGG GAG GCC TCC TCC GAG CGG ATG TAC CCC GAG GAC GGC GCC CTG AAG GGC GAG ATC AAG CAG AGG CTG AAG CTG AAG GAC GGC GGC CAC TAC GAC GCT GAG GTC AAG ACC ACC TAC AAG GCC AAG AAG CCC GTG CAG CTG CCC GGC GCC TAC AAC GTC AAC ATC AAG TTG GAC ATC ACC TCC CAC AAC GAG GAC TAC ACC ATC GTG GAA CAG TAC GAA CGC GCC GAG GGC CGC CAC TCC ACC GGC GGC ATG GAC GAG CTG TAC AAG TAA GCG GCC GCC AGG CCA GGA TGT CCA GTC GCC CGT GAG CCG GGG GGC ACG GGC TGG AAG AAG TAG CGA TGG ACG AGC GGC CCG CCA GCC CCG ACG CTG CCC AGG CCG TCC TCC CCA ACA ATA TCC TGG TGC TGA GTG GGT GCA CAG TGA CTC CAG CAT CAG TGA TTT TGT TGA AGA GGG CAG CTG CCA GCC TCC TGT CCT GCC TGA TGC CTA GCC CAG CTG CCC CAC CCA TAG GTC ACC CAA CCC CGC ATC CGT CCC CTG GTA CAG TCC TCC TGG GAC GCG T -3’

*The underline in the 5’ end is the Agel restriction site, the underline in the 3’ end is the NheI restriction site. Black bold font indicates the target gene sequence. **The underline in the 5’ end is the AgeI restriction site, the underline in the 3’ end is the MluI restriction site. Black bold font indicates the target gene sequence. miR: microRNA; Pre: precursor.