Figure 2.

Macrostructure and components of a DNM-S.

(A, B) Appearance of an intact DNM-S. Black asterisk indicates the nerve endoneurium, and black arrow indicates the perineurium-epineurium. The nerve endoneurium area is semi-transparent and loose, while the perineurium-epineurium is white and relatively dense. (C) Sirius red image of cross-sectional a DNM-S. Black asterisk indicates the nerve endoneurium component, and black arrow indicates the perineurium-epineurium component. (D, E) The perineurium-epineurium contains a cylinderical sheath structure corresponding to the black arrows in (A) and (B). (F) The fasciculus (endoneurium) contains a longitudinal strands structure. Scale bars: 1 mm in A, B, D–F. (G) Characterization of total number of proteins detected in DNMS tissue, categorized into core matrisome proteins. The listed proteins are the expressed proteins identified in DNM-S, grouped into clusters according to their function. (H) Histograms showing the number of proteins in different kinds of ECM. DNM-S: Decellularized nerve matrix scaffold; ECM: extracellular matrix.