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Lnc-ZFAS1 facilitates cell proliferation and represses apoptosis in osteosarcoma. For functional investigation, U2OS and KHOS cells were respectively infected with Lnc-ZFAS1 overexpression and Lnc-ZFAS1 shRNA plasmids, and qRT-PCR was performed for examination of transfected efficacy (A). Colony formation (B and D) and EdU (C and E) assays were utilized for proliferation analysis. (F) Western blotting was adopted to examine the expression of apoptosis-related proteins (**p < 0.01).
