Skip to main content
. 2022 Dec 5;78:100143. doi: 10.1016/j.clinsp.2022.100143

Figure 4.

Figure 4

Lnc-ZFAS1 sponges miR-520b and miR-520e in osteosarcoma. (A) The subcellular location of Lnc-ZFAS1 was determined. GAPDH and U6 were respectively determined as the markers of cytoplasm and nucleus. (B) FISH results show Lnc-ZFAS1 (in green) cellular distribution in KHOS cells. (C) Starbase and GSE28423 databases were performed to obtain intersected hsa-miR-520b, hsa-miR-520e and hsa-miR-934. RNA pull-down (D), Luciferase reporter (E) and RIP (F) assays were utilized to explore the binding between Lnc-ZFAS1 with two miRNAs. (G) miR-520b and miR-520e were detected in Lnc-ZFAS1-upregulated U2OS cells and Lnc-ZFAS1-downregulated KHOS cells by qRT-PCR. (H) qRT-PCR was employed to detect the expression of miR-520b and miR-520e in osteosarcoma tissues and normal tissues (n = 8). (I) The correlation analysis between Lnc-ZFAS1 with two miRNAs (*p < 0.05, **p < 0.01).