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. 2022 Nov 25;66:101645. doi: 10.1016/j.molmet.2022.101645

Figure 2.

Figure 2

Electrophysiological properties of POMC neurons from PomcCreand Stim1Mutmale mice. A, Resting membrane potential (RMP). B, Cell capacitance (Cm). C, Input resistance (Rin). Unpaired t-test, t(49) = 2.903, ∗∗p = 0.0055, PomcCre, n = 25; Stim1Mut, n = 26. D, Rheobase. E, firing frequency. There were significant differences in the evoked firing rate between PomcCre and Stim1Mut groups (two-way ANOVA: main effect of treatment, F(1,24) = 4.464, p = 0.0452, main effect of current, F(8,192) = 108.7, p < 0.0001, and interaction, F(8,192) = 3.223, p = 0.0018; PomcCre, n = 13, Stim1Mut, n = 13; post hoc Bonferroni test, ∗p < 0.05 and ∗∗p < 0.01). F, overshot. Unpaired t-test, t(30) = 3.127, ∗∗p = 0.0039, PomcCre, n = 18; Stim1Mut, n = 14. G, full width at half maximum (FWHM). Unpaired t-test, t(30) = 2.232, ∗p = 0.0322, PomcCre, n = 18; Stim1Mut, n = 14. H, Peak to afterhyperpolarization (AHP) amplitude. Unpaired t-test, t(25) = 2.356, ∗p = 0.0266, PomcCre, n = 17; Stim1Mut, n = 10. I, threshold. J, AHP amplitude. K, AHP duration. L, Phase plots of elicited action potentials.