Fig. 1.

Changes in FABP3, FABP5, and FABP7 expression following ischemic stroke. (A) Differentially expressed FABP genes in the blood of acute ischemic stroke patients (n = 23) were graphed in a volcano plot, with the log₂ fold change on the X-axis and the -log₁₀ P-value on the Y-axis. Red dots represent significantly upregulated mRNA and grey points represent non-significant mRNA. (B) Changes of FABP3, FABP5, and FABP7 mRNA in whole blood within 24 h after reperfusion in tMCAO/R rats (n = 8). (C) Schematic representation of tissues used for FABP quantification. The non-ischemic contralateral (green) and ischemic ipsilateral (pink) hemispheres were analyzed. (D) Mice were subjected to tMCAO/R. Brain tissues were collected 6, 12, 24, and 48 h after reperfusion for Western blot analysis of FABP levels. (E) Schematic diagram of the area where the immunofluorescence staining results were collected, which is marked with an asterisk (*). (F) Representative immunofluorescence staining of FABP3, FABP5, or FABP7 (green), NeuN (a neuronal marker, red), GFAP (an astrocyte marker, red), and DAPI (nuclei, blue) in sham and I/R mice. Scale bar = 100 μm. (G) The proportion of FABP3- or FABP5-expressing NeuN-positive neurons and FABP7-expressing GFAP-positive astrocytes. The proportion of FABP3-, FABP5- and FABP7-positive cells that were neurons or astrocytes is also shown. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)