Fig. 2.
Effect of HY08 on FABP expression in I/R mice. (A) Chemical structure of HY08. (B) ANS displacement assay of HY08 derivatives and GST-FABPs. The fluorescent probe ANS (4 μM) was mixed with GST-FABP in the presence 0, 100, 1000, 2000, or4000 nM HY08. The fluorescence intensity was measured at 460 nm. (C) The Kd values of HY08 to FABPs were measured via ANS assay. (D) Representative Western blots. I/R mice were treated with 0.3 mg/kg MF6 at 30 min after reperfusion. 12 h after reperfusion, brain tissues were used for Western blot analysis of FABP levels. Quantitative analyses of FABP3 (E), FABP5 (F), and FABP7 (G) protein expression levels in contralateral and ipsilateral brain tissues. The data shown in each column represent the mean ± SEM. *p < 0.05, **p < 0.01 vs. the vehicle-treated sham group (contralateral/ipsilateral); #p < 0.05, ##p < 0.01 vs. the vehicle-treated I/R group (contralateral/ipsilateral) (n = 6 per group).