Fig. 8.

FABP5 is involved in BAX and VDAC-1 oligomerization in SH-SY5Y cells. (A) Representative immunofluorescence staining of BAX (green), Mito-tracker (red), and FABP5 (blue) in rotenone-treated SH-SY5Y cells. Scale bar = 50 μm. (B and C) Representative Western blots showing BAX protein levels in mitochondrial fractions (B). Quantitative analysis of BAX oligomers (C) in SH-SY5Y cells following rotenone stimulation. (D–F) Representative Western blots (D) and quantitative analyses of cytochrome c (E) and cleaved caspase-3 (F) in cytosolic fractions. (G and H) Representative Western blots showing VDAC-1 protein levels in mitochondrial fractions (G). Quantitative analysis of VDAC-1 oligomers (H) in SH-SY5Y cells. The data shown in each column represent the mean ± SEM. **p < 0.01 vs. vehicle-treated mock cells; ^#p < 0.05, ^##p < 0.01 vs. rotenone-treated mock cells. (n = 4). (I) Schematic diagram of the involvement of FABP5 in the formation of BAX and VDAC-1 channels in the outer mitochondrial membrane during oxidative stress. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)