Figure 2:

Comparative microanatomical analysis of lung identifies lasting structural derangement.

a) Clustered heatmap of pairwise correlation between images based on the abundance of cell types. Image group identity is marked by colors in rows and columns with legend in b). b) Two-dimensional projection of the same data as in a) using Spectral Embedding. c) Same as a) but grouped by disease group. d) Scatter plot of effect sizes estimated from comparing various disease groups. Point size is related to the significance of change, while color displays agreement between comparisons (agreement in red, disagreement in blue tones). e) Representative images of macrophages in acute COVID and PASC. f) Image highlighting vascular network and muscularization of lung tissue in PASC sample. g) Absolute abundance of smooth muscle cells in lung tissue from various disease groups. h) Exemplary image of blood clot where MPO⁺ neutrophils can be seen in the lumen with Citrullinated H3 from NETs in proximity. i) Quantification of extracellular citrullinated H3 signal in healthy lung and PASC. j) Scatter plot of estimated effect sizes of cell type abundance changes compared between patients in the PC-pos and -neg groups (x-axis), and within PC patients along the time since disease onset (y-axis). k) Absolute abundance of mast cells in the lungs of patients from the various disease groups. l) Representative images of secreted CC16 for various disease groups. AQP1 and Collagen shown as reference. m) Amount of extracellular CC16 (SCGB1A1) across disease groups for images containing airways.