FIGURE 1:

TrimAway IP₃R in Xenopus oocytes. (A) Control oocytes and oocytes injected with mCherry-Trim21 mRNA were incubated for 24 h followed by injection with anti-IP₃R antibodies, as indicated. Oocytes were lysed 4 h after antibody injection for immunoblotting, first using anti-IP₃R antibodies followed by and anti-β-tubulin antibodies. Molecular weight markers are shown. Arrow depicts IP₃R. Representative of three independent experiments. (B) Control oocytes and oocytes injected with eGFP-Trim21 mRNA were incubated for 24 h followed by injection with anti-IP₃R antibodies or anti-GST antibodies, as indicated. Four hours following antibody injection, oocytes were lysed for immunoblotting using anti-IP₃R antibodies or anti-tubulin antibodies. Arrow depicts IP₃R. Representative of three independent experiments.