FIGURE 2:

Depletion of the COPI complex induces the formation of LDs. PC3, U2OS, or DU145 cells were transfected with siCONT, siCOPA, and siCOPZ1; 72 h posttransfection, LDs and nuclei were visualized with BODIPY 493/503 (green) and DAPI (blue), respectively. (A). Fluorescent images of LDs in transfected cells. (B–D). Quantification of the effects of COPI complex depletion on the number and size of LDs in DU145 (B), PC3 (C), and U2OS (D) cells transfected with siRNAs. Plots in the left panels represent the mean of the number of the droplets per cell ± SEM. The total number of LDs was quantified from fluorescent images of triplicate experiments (∼5 fields per experiment, 50–100 cells per data point). Plots in the middle panel represent the mean of droplet areas in transfected cells ± SEM. The areas of the LDs (500–1300) per data point were obtained from three independent transfections. Plots in the right-hand panel show the distribution of droplet size in the transfected cells. Distributions are presented in a logarithmic scale. Scale bar = 25 μm.