FIGURE 3:

MitoQ and JNK inhibitor SP600125 decrease the size of LDs induced by depletion of COPI complex proteins. Cells were transfected with siCONT, siCOPA, and siCOPZ1 in triplicates. 48 h posttransfection cells were treated with MitoQ, JNK inhibitor, or vehicle control (DMSO) for an additional 24 h. (A). Representative fluorescent images of LDs (green) and nuclei (blue) visualized by BODIPY and DAPI staining, respectively, in the untransfected (control) and siRNA-transfected PC3 cells treated with JNK inhibitor, MitoQ, or vehicle (DMSO). Images of DU145 and U2OS cells are shown in Supplemental Figure S4, A and B. (B) Plots represent the mean of droplet areas in transfected cells ± SEM. (C) Plots represent the mean of the number of the droplets per cells ± SEM. Scale bar = 25 μm.