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. 2022 Nov 18;33(14):ar135. doi: 10.1091/mbc.E21-08-0420

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© 2022 Gasparian et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Time course of the decrease size of LDs in COPI-depleted cells by MitoQ treatment. PC3 cells were transfected with siCOPA; 48 h posttransfection, cells were treated with MitoQ or vehicle control up to 24 h. The cells were fixed at seven time points (0, 2, 4, 8, 12, and 24 h) followed by LD staining with BODIPY. (A) BODIPY staining of MitoQ-treated or vehicle-treated COPI-depleted cells. (B) Analysis of droplet size distribution. Plots show size distribution. The droplet area is shown in log10 scale. (C) Plot represents maximums of droplet size distribution over time of MitoQ treatment. Scale bar = 10 μm.