FIGURE 5:

COPI depletion does not affect the rate of lipolysis and inhibition of lipolysis results in droplets insensitive to ROS depletion. (A) Representative immunofluorescence images of LDs in PC3 cells were treated with 100 µM Atglistatin for 48 h, ± MitoQ, JNK inhibitor, or DMSO (vehicle). LDs are visualized by BODIPY (green) and nuclei by DAPI (blue) staining. (B) Plot represents the average size of the LDs in the treated cells ± SEM. (C) Plot showing the release of [³H]-NEFA into the medium from cells treated with Atglistatin or transfected with siCOPA ± MitoQ or JNK inhibitor. The bars are the average of quadruplicates ±SEM. Scale bar = 40 μm.