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. 2022 Nov 18;33(14):ar135. doi: 10.1091/mbc.E21-08-0420

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FIGURE 6: — Depletion of the COPI elevates expression of markers of autophagy and number of LDs colocalized with autophagy markers. (A) Western blotting analysis of the expression of LC3 and p62/SQSTM1 in COPZ1 or COPA-depleted cells. (B) Western blotting analysis of Atg5 expression in PC3, U2OS, and DU145 cell lines. (C) Representative immunofluorescent images of LDs and LC3 in the control or siRNA-transfected PC3 cells; 72 h posttransfection, the LC3 was visualized by staining with specific antibodies (red), LDs by BODIPY (green), and nuclei by DAPI (blue) staining. Images of U2OS and DU145 cells are presented in Supplemental Figure S5. Scale bar = 25 μm. (D) Quantification of LC3-positive LDs. The total number of LDs and the number of droplets positive for LC3 were determined. Plot represents the average quantification of at least 12 different fields from three independent transfections, ± SEM.