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. 2022 Nov 18;33(14):ar135. doi: 10.1091/mbc.E21-08-0420

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© 2022 Gasparian et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 8: — EM analysis of LDs in COPI knockdown cells did not reveal any accumulation of double-membrane autophagosomes. PC3 cells were transfected with siCONT or siCOPA for 48 h, then treated with MitoQ or vehicle control, followed by fixation at 72 h posttransfection. The structure of the droplets was analyzed by TEM with either low (7,000× or 8,000×) or high (12,000×) magnifications. Panels B and D show parts of panels A and C, respectively, at the higher magnifications. Panels E and F show separate areas taken at different magnifications. LDs = lipid droplets. Scale bar = 0.5 μm.