FIGURE 1:

Lgd localizes to endosomes during early C. elegans embryogenesis. (A) Representative images taken in utero of animals natively expressing a GFP fusion to Lgd using confocal microscopy are shown at 30-s intervals (n = 6 animals). Bar, 10 μm. (B, D) Representative images of zygotes coexpressing a GFP fusion to Lgd and a mCherry fusion to Rab5 (B) or STAM (D) were acquired live using confocal microscopy (n = 6 animals each). Bars, 10 μm. (C, E) Quantification showing the percentage of Lgd-labeled structures that also harbor Rab5 (C) or STAM (E), calculated using Imaris.