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Loss of Lgd impairs ILV formation within MVEs. (A) Representative images of MVEs in control and lgd mutant zygotes following negative staining electron microscopy (n = more than 60 MVEs, each). Bar, 100 nm. (B–D) Quantification of MVE (C) and ILV (D) diameter in control and lgd mutant zygotes, including a table with averages (B). *, p < 0.05, based on a t test. (E) Histogram showing the relative number of ILVs identified in thin sections from control and lgd mutant zygotes.
