FIGURE 2:

KIF3AB and KIF3AC dimerization does not require the canonical N-terminal dimerization domain. (A) Schematic showing the construct design. (B) Schematic illustrating the specific application of the protein–protein binding assay. (C, D, F–H) Representative images of 9 DIV neurons expressing the indicated proteins and treated with linker. High-magnification images of dendrites were taken from the regions indicated by the yellow line. Arrowheads highlight examples of colocalization. The inset in H shows GFP-KIF3C aggregates in the soma. Low-magnification scale bar: 20 µm. High-magnification inset scale bar: 5 µm. (E, I) Quantification of colocalization between peroxisomes and kinesins. Cell sample sizes, panel E: KIF3B(–): 10; KIF3B(+): 16; KIF3C(–): 9; KIF3C(+): 11. Cell sample size, panel I: KIF3A(–): 11; KIF3A(+): 10; KIF3B(–): 14; KIF3B(+): 16; KIF3C(–): 7; KIF3C(+): 17. ***p < 0.001.