FIGURE 3:

KAP binding to KIF3 is not mediated by coiled-coil domains. (A) Schematic showing the construct design. (B) Schematic illustrating the specific application of the protein–protein binding assay. (C, D, F–H) Representative images of 7 DIV neurons expressing the indicated proteins and treated with linker. High-magnification images of dendrites were taken from the regions indicated by the yellow line. Scale bar: 20 µm. (E, I) Quantification of colocalization between peroxisomes and kinesins. Cell sample sizes, panel E: KIF3AB^CC(–): 12; KIF3AB^CC(+): 15; KIF3AC^CC(–): 14; KIF3AC^CC(+): 15. Cell sample sizes, panel I: KIF3A^CC(–): 13; KIF3A^CC(+): 14; KIF3B^CC(–): 13; KIF3B^CC(+): 14; KIF3C^CC(–): 13; KIF3C^CC(+): 14. n.s. not significant.