FIGURE 4:
KAP binds the KIF3 C-terminal disordered domain. (A) Schematic showing the construct design. (B) Schematic illustrating the specific application of the protein–protein binding assay. (C, D, F–H) Representative images of 7 DIV neurons expressing the indicated proteins and treated with linker. High-magnification images of dendrites were taken from the regions indicated by the yellow line. Arrowheads highlight examples of colocalization. Scale bar: 20 µm. (E, I) Quantification of colocalization between peroxisomes and kinesins. Cell sample sizes, panel E: KIF3ABDD(–): 15; KIF3ABDD(+): 15; KIF3ACDD(–): 15; KIF3ACDD(+): 15. Cell sample sizes, panel I: KIF3ADD(–): 15; KIF3ADD(+): 15; KIF3BDD(–): 14; KIF3BDD(+): 16; KIF3CDD(–): 12; KIF3CDD(+): 15. ***p < 0.001.