FIGURE 5:

Characterization of KIF3B, KIF3C, and KAP organelles in cultured hippocampal neurons. (A) Schematic showing the construct design. (B–D) Representative images of 7 to 8 DIV hippocampal neurons expressing the indicated proteins. GFP-KIF3B and GFP-KIF3C were coexpressed with untagged KIF3A tail. Halotag was visualized with JF549. Yellow lines on each cell indicate sections of the axon and dendrite from which the high-magnification views and kymographs were generated. Scale bars: 20 µm. (E–G) Quantification of organelle motility and transport events. Sample sizes: KIF3B: 15 cells, 1027 axonal and 4741 dendritic organelles; KIF3C: 15 cells, 1090 axonal and 4747 dendritic organelles; KAP: 14 cells, 1001 axonal and 4470 dendritic organelles. *p < 0.05; ***p < 0.001.