TABLE 1:
Model parameters and MT sliding quantification in computational model.
| Parameter | Description | Value | References or comment |
|---|---|---|---|
| EI | Flexural rigidity per doublet | 73 pN·μm2 | Okuno and Brokaw, 1979; Pelle et al., 2009; Xu et al., 2016 |
| kN | Interdoublet normal stiffness | 105 pN/μm2 | Yagi and Kamiya, 1995; Minoura et al., 1999; Xu et al., 2016 |
| kN_RS | RS normal stiffness | 105 pN/μm2 | Found to sufficiently maintain axoneme cross-section shape without convergence issues |
| kT_RS | RS shear stiffness | 100 pN/μm2 | Estimate found to allow sufficient range of motion |
| kN_AC | AC-linker normal stiffness | 106 pN/μm2 | Estimate found to give triplet separation and curvature independence consistent with data |
| kT_AC | AC-linker shear stiffness | 105 pN/μm2 | Estimate found to give triplet translocations consistent with data |
| cN, cT | Normal, tangent resistive force coefficient | (3, 1.5) × 10–3 pN·s/μm2 | Gray and Hancock, 1955; Bayly et al., 2011 |
| kCortex | Cortical attachment stiffness per filament | 11 pN/μm | Estimate found to allow BB bending consistent with data |
| Lc | Cilium length | 5.6 μm | Akella et al., 2010; Junker et al., 2021 |
| Lbb | BB length | 500 nm | Allen, 1969 |
| L_SF | SF horizontal length | 1.2 μm | Soh et al., 2020 |
| L_pcMT | Postciliary MT horizontal length | 500 nm | This study |
| L_tMT | Transverse MT horizontal length | 187 nm | This study |
| L_AC | Axial length of AC-linkers | 150 nm | Meehl et al., 2016 |
| L_CW | Axial length of CW | 75 nm | Meehl et al., 2016 |
| r | Axoneme radius | 100 nm | Nicastro et al., 2006; Lindemann and Mitchell, 2007 |
| P | Distributed dynein force | 500 pN/μm | Shingyoji et al., 1998 |
| T | Period of oscillation | 40 ms | Soh et al., 2022 |
| pLag | Phase lag between adjascent BBs | 3 ms | Soh et al., 2022 |
| Maximum relative sliding (nm) for MT pair: | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1–2 | 2–3 | 3–4 | 4–5 | 5–6 | 6–7 | 7–8 | 8–9 | 9–1 | |
| Cilium tip | 136 | 147 | 106 | 8 | 109 | 151 | 137 | 61 | 62 |
| Cilium base | 19 | 25 | 21 | 6 | 12 | 21 | 21 | 12 | 7 |
| Base - 10× less stiff AC-linker | 23 | 29 | 23 | 8 | 12 | 25 | 24 | 12 | 11 |
| Difference from WT | 21% | 14% | 10% | 33% | 0% | 19% | 14% | 0% | 57% |
Computational model parameters and data for the relative sliding between adjacent MTs at tip and base of modeled cilium and BB. For relative sliding values, row 3 gives relative sliding at the cilium base when the model is perturbed by reducing AC linker stiffness by a factor of 10. Row 4 gives the percent difference in sliding magnitudes at the base of the cilium in the perturbed model.