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. 2022 Nov 18;33(14):ar138. doi: 10.1091/mbc.E21-11-0540

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© 2022 Rivera et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 1: — Ovarian cancer cell models. Ovarian cancer cell lines with Rac1 OE or KD were generated to investigate the role of Rac1 in ovarian cancer metastasis. (A, B) Quantification of endogenous Rac1 expression in SKOV3ip, OVCAR3, and OVCAR8 cell lines. SKOV3ip cells have two- and threefold increases in endogenous Rac1 expression compared with OVCAR3 and OVCAR8 cells, respectively. (C, D) Quantification of Rac1 expression in SKOV3ip ovarian cancer cells. (E, F) Quantification of Rac1 KD by CRISPR-Cas9 in SKOV3ip ovarian cancer cell clones that were selected after CRISPR electroporation via single-cell cloning. Means ± SD (n = 2 for B, D, and F).