FIGURE 3:

Rac1 is important for ovarian cancer cell invasion. Invasion of SKOV3ip ovarian cancer cells was assessed using Matrigel Transwell inserts. SKOV3ip cells with GFP or increasing levels of GFP-Rac1 overexpression (GFP-Rac1 Low and GFP-Rac1 High) or Rac1 CRISPR-Cas9 KD were compared. (A) Representative images of SKOV3ip GFP control and GFP-Rac1 OE cellular invasion. (B) Quantification of the number of invaded ovarian cancer cells after 48 h. (C) Representative images of SKOV3ip Rac1 CRISPR-Cas9 KD cellular invasion. (D) Quantification of ovarian cancer cell invasion after 48 h. Quantification is based on three or four independent experiments with three technical Transwell insert replicates per cell line. Five random fields of view were imaged, counted, and averaged for each insert. Means ± SD (n = 3 in B, n = 4 in D). One-way ANOVA: ***p < 0.0002, ****p < 0.0001.