FIGURE 4:

Rac1 OE decreases area of SKOV3ip spheroids. SKOV3ip cells Parental, GFP control, GFP-Rac1 OE, Rac1 CRISPR-Cas9 control, Rac1 CRISPR-Cas9 KD clone 23, and Rac1 CRISPR-Cas9 KD clone 27 were seeded in U-bottom plates to form spheroids (100 cells/well). Spheroids were imaged in bright field after 4 ds of incubation. (A) Representative images of spheroid formation for each cell line. (B) Quantification of spheroid area comparing every cell line to each other. Means ± SD (n = 19 in Rac1 CRISPR-Cas9 control, and n = 20 in the rest of the cell lines). One-way ANOVA, followed by Tukey’s test: ****p < 0.0001. Scale bar is 200 μm.