FIGURE 7:

OCMetSim-Single Cells model predicts that Rac1 OE contributes to tumor cell metastasis to distant metastatic niche sites. (A) Single-cell simulation results comparing the number of cells that reach a distant metastatic niche with Rac1 OE or KD. Using the parameters for increased invasion and adhesion with Rac1 OE, and varying probabilities of cell death in the blood, these results demonstrate that Rac1 OE results in more ovarian cancer cells reaching a distant metastatic niche in less time, compared with two Rac1 CRISPR-Cas9 KD clones (clones 23 and 26). (B) Representative image of 100 runs with 20% death in the blood and parameters for Rac1 OE or Rac1 CRISPR-Cas9 KD, displaying the rate at which the cells enter the distant niche site. The blue line represents the decrease in the number of cells in the peritoneal compartment for Rac1 OE and KD cells over time. (C) The slope of each cell type entering the distant niche site was quantified to determine the rate at which cells entered the distant niche site. Rac1 OE cells have a higher rate at which they enter the distant niche site compared with two Rac1 CRISPR-Cas9 KD clones. Thirty simulations were run for each experiment. Error bars represent means ± SD. Two-way ANOVA followed by Tukey’s post-hoc test for multiple comparison across columns and rows was used to compare differences between cell type and probability of death in the blood: **p < 0.01, ****p < 0.0001.