TABLE 1:

OCMetSim-Single Cells and OCMetSim-Spheroids parameters.

Experimental parameters	Initial number of cells	Cell line	Probability of adhesion calculated from ECIS data	Probability of adhesion calculated from mesothelial clearance assay	Rate of invasion calculated from Matrigel experiments (×1000)	Rate of invasion determined from Matrigel/mesothelial clearance experiments	Probability of death in the bloodstream	Probability of death from crowding
Single cell model	1000 or 5000	Rac1 OE cells	80%	N/A	0.060578704	N/A	Varied between20, 50, and 80%	Varied between0 and 50%
		Rac1 CRISPR-Cas9 KDclone 26; KD cells	42%	N/A	0.001757813	N/A	Varied between20, 50, and 80%	Varied between0 and 50%
		Rac1 CRISPR-Cas9 KDclone 23; KD cells	36%	N/A	0.000277778	N/A	Varied between20, 50, and 80%	Varied between0 and 50%
Spheroid model	111	Rac1 OE cells	N/A	100%	N/A	0.001	Varied between20, 50, and 80%	Varied between 0 and 50%
		Rac1 CRISPR-Cas9 KDclone 23; KD cells	N/A	100%	N/A	0.0002	Varied between20, 50, and 80%	Varied between0 and 50%

Summary of the parameters used for Rac1 OE and KD cell lines. The parameters were used to obtain the data displayed in Figures 7–10.