TABLE 2:

Agent-based modeling simulation results, summarizing the main assumptions and findings from the computational experiments that were performed.

	Single cell or spheroid	Cell crowding?	Figure	Main assumptions	Results
Experiment 1;1000 cells	Single cell	No	Figure 7, A–C	Cells died if they did not adhere	1) Rac1 OE increased the number of cells that reached the distant tissue site compared with Rac1 CRISPR-Cas9 KD2) Rac1 OE cells reached the distant tissue site faster than Rac1 CRISPR-Cas9 KD cells
Experiment 2;1000 cells	Single cell	Yes	Figure 8, A and B	1) Cells died if they did not adhere2) Cells died if crowding prevented adhesion	1) Cell death from crowding reduced the number of KD cells that reached the distant site but not the number of Rac1 OE cells2) More Rac1 OE cells reached the distant site compared with Rac1 KD3) Rac1 OE cells reached the distant site faster than Rac1 KD
Experiment 2;5000 cells	Single cell	Yes	Figure 8, C and D	1) Cells died if they did not adhere2) Cells died if crowding prevented adhesion	1) Cell death from crowding reduced the number of Rac1 OE and KD cells that reached the distant site2) More Rac1 OE cells reached the distant site compared with Rac1 KD4) Rac1 OE cells still reached the distant site faster than Rac1 KD
Experiment 1a	Spheroid	No	Figure 9, A–B	1) 100% spheroid adhesion, no spheroid death2) Spheroids died if crowding prevented adhesion	1) The numbers of Rac1 OE and KD spheroids that reached the distant site were the same2) Rac1 OE spheroids reached the distant site faster than Rac1 CRISPR-Cas9 KD spheroids
Experiment 2a; 100% adhesion	Spheroid	Yes	Figure 9, C and D	1) 100% spheroid adhesion, no spheroid death2) Spheroids died if crowding prevented adhesion	1) Cell death from crowding reduced the number of Rac1 OE and KD spheroids in the distant site2) Rac1 OE spheroids reached the distant site faster than Rac1 CRISPR-Cas9 KD spheroids