| Types of metabolites | Sampling solution composition | Temperature of extraction | Duration of extraction | Evaporation | Solution of resuspension |
|---|---|---|---|---|---|
| Central metabolites | ACN(4)/MeOH(4)/ H2Omq (2) 125 mM formic acid | −20°C | 1 h minimum | SpeedVac | Water |
| Intracellular amino acids | ACN(4)/MeOH(4)/ H2Omq (2) 125 mM formic acid | −20°C | 1 h minimum | SpeedVac | Water |
| Coenzyme A | ACN(4)/MeOH(4)/ H2Omq (2) 125 mM formic acid | ‐20°C | 1 h minimum | SpeedVac | 2% methanol, 98% water, 25 mM formic acid, pH adjusted at 3.5 |
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