Extended Data Figure 9. Colonization of Veillonella in a mouse model of colitis.

a) Raw Ct values of the Veillonella 16S rRNA gene measured by qPCR in the stool of WT-colonized ASF mice with and without DSS treatment (n=8–11 mice). b) Absolute abundance of Veillonella in the stool of ASF mice as measured by CFU per mg of stool on selective SKLN media (n=3 mice). c) Absolute abundance of Veillonella in the colonic tissue of ASF mice as measured by CFU per mg of tissue on selective SKLN media (n=4 mice). d) Concentration of lactate in the gut of control- and DSS-treated ASF mice colonized with WT Veillonella (n=8 mice). e) Relative expression of the IIdD, lldP, hisA and argB genes in WT Veillonella colonizing the stool of mice with and without DSS treatment (n=3–4). The gyrA gene was used as an internal control. f) Colon lengths of WT and iNOS KO SPF mice (n=4–7). Data represent two independent experiments and show mean (a, d-f) ± SEM (d-f) or median (b,c) values. Data were analyzed by one-way ANOVA and Tukey’s HSD test or Mann-Whitney U-test.