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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Nat Microbiol. 2022 Sep 22;7(10):1673–1685. doi: 10.1038/s41564-022-01224-7

Figure 2. Nitrate respiration expands the metabolic repertoire of Veillonella.

Figure 2.

WT growth in a) SKN lacking casitone or yeast extract (n=3) and b) SK base (SKb) with or without nitrate and increasing concentrations of casamino acids, casitone, bacto peptone, or tryptone (n=4). c) Amino acid composition in uninoculated SK (n=6) and spent SKL, SKN, and SKLN (n=3) media at mid- and late exponential phases. d) WT growth in SKb plus nitrate (SKbN) and SKb with or without the indicated amino acids (25mM) (n=4). e) Dipeptide consumption in uninoculated SK (n=6) and spent SKL, SKN, and SKLN (n=3) media at the late exponential phase. f) WT (n=12), oppABC (n=12), and optAB (n=11) growth in SKbN with or without the indicated dipeptides (20 mM). Growth rates (μmax) were calculated for each growth curve (d,f). Data (c-f) represent mean±SEM, analyzed by one-way ANOVA and Tukey’s HSD test. NS, not significant. N numbers denote biological replicates.