Fig. 2.

Four channel high content imaging analysis: A) cell counts were based on nucleus staining (Hoechst) and used as the overlay for calculating the percentage of positive cells for B) FOXA2 expression, C) mitochondrial activity, or D) cell viablilty for each timepoint and treatment. Error bars indicate standard error for three well replicates (Cell Profiler v2.2.0). E) Representative images of Hoechst (blue) and FOXA2 (red) co-expression (purple) for each treatment. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).