Fig. 3. The region upstream of leuO is bound by H-NS.

DNaseI protection assay of the region upstream to leuO using purified H-NS. 200 ng of labeled leuO promoter DNA was combined with either no H-NS, lanes 1 and 6; or 0.5 μM, 1 μM, 2 μM, and 5 μM H-NS, lanes 2-5, respectively. The nucleotide sequences protected by H-NS indicated as blue letters and those protected by LeuO boxed in red color are indicated on the genetic map.