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. 2020 Aug 28;30(11):1614–1625. doi: 10.4014/jmb.2006.06042

Fig. 6. TMEE promotes the nuclear translocation of Nrf2 and the induction of HO-1 in RAW 264.7 cells.

Fig. 6

(A) The protein level of Nrf2 in nucleus was measured using western blot analysis. (B) The expression level of HO-1 in whole lysate was determined using western blot analysis. (C) The mRNA levels of HO-1 and NQO1 were determined using RT-PCR. Quantitative analysis of (D) HO-1 and (E) NQO1 levels was performed using densitometric analysis. Data are expressed as the mean ± standard deviation. *p < 0.05 and **p < 0.01 vs. negative control group. Nrf2, nuclear translocation of nuclear factor erythroid-2-related factor 2; TMEE, Trichilia martiana C. DC ethanol extract; HO-1, heme oxygenase-1; NQO1, NAD(P)H quinone oxidoreductase 1.