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Biotransformation in engineered E. coli. Engineered E. coli produce glycosyltransferases (GTs) and nucleoside diphosphate sugar (NDP-sugar). The NDP-sugar is used by GTs for the formation of quercetin glycosides. On the other hand, O-methyl transferase (OMTs) uses S-adenosylmethionine (AdoMet) for methylation of quercetin releasing S-adenosylhomocysteine (AdoHcy).
